Journal:

Article Title: Comparison of a New Quantitative ompA -Based Real-Time PCR TaqMan Assay for Detection of Chlamydia pneumoniae DNA in Respiratory Specimens with Four Conventional PCR Assays

doi: 10.1128/JCM.41.2.592-600.2003

Figure Lengend Snippet: Microorganisms a used to test the specificity and cross-reactivity of the real-time PCR assay designed to detect the ompA gene of C. pneumoniae

Article Snippet: C. trachomatis , ATCC (VR-902B).

Techniques: Real-time Polymerase Chain Reaction

Journal:

Article Title: Comparison of a New Quantitative ompA -Based Real-Time PCR TaqMan Assay for Detection of Chlamydia pneumoniae DNA in Respiratory Specimens with Four Conventional PCR Assays

doi: 10.1128/JCM.41.2.592-600.2003

Figure Lengend Snippet: ClustalW multiple-sequence alignments of the 85-bp PCR product (QM85), primers (forward primer, QMOMP1; reverse primer, QMOMP2) and probe (QMOMPS) of the real-time PCR assay for the detection of the ompA genes of C. pneumoniae (row 1, MOMP [AF131889]; row 2, MOMP [AF131230]; row 3, MOMP [AF131229]; row 4, IOL-207, CHTMOMPEB [M64064]; row 5, J138, ompA [NC_002491]; row 6, CWL-029, ompA [AE001363]; row 7, AR39, MOMP [AE002167]; row 8, CHTMOMPP [M69230]; row 9, koala type I, CHTOMPAAI [M73038]; row 10, CHTMOMPEQ [L04982]), C. suis (row 11), PCLH197, ompA [AJ440241]), C. psittaci (row 12, strain 84-55, omp1 [Y1656]; row 13, 6BC, MOMP [56980]), C. trachomatis (row 14, C/TW3/OT, omp1 [AF352789]), C. trachomatis (row 15, strain D/Ep6, omp1 [X77364]), C. felis (row 16, Fpn/pring, CPFPNMOMP [X61096]), C. muridarum (row 17, SFPD, CHTMOMPZ [L19221]), C. caviae (row 18, ATCC VR813, ompA [AF269282]), C. pecorum (row 19, LW613, ompA [AJ440240]), and C. abortus (row 20, LLG, omp1 [AF272945]) (the designations in brackets are GenBank accession numbers). Dots and empty spaces indicate identities and gaps compared with the target sequence of the C. pneumoniae strain with GenBank accession no. AF131889, respectively.

Article Snippet: C. trachomatis , ATCC (VR-902B).

Techniques: Sequencing, Real-time Polymerase Chain Reaction

Journal: Viruses

Article Title: Assembly and Capsid Expansion Mechanism of Bacteriophage P22 Revealed by High-Resolution Cryo-EM Structures.

doi: 10.3390/v15020355

Figure Lengend Snippet: Figure 1. The cryo-EM structure of the P22 procapsid. (A) The overall structure of the procapsid. The seven subunits of the procapsid asymmetric unit are in different colors. (B) The segmented asymmetric unit from the local reconstruction of procapsid. (C) Accurate atomic models based on the density map in panel B. (D) Density maps of gp5 at 2.6 Å resolution superimposed on their atomic models. The gp5 structure is divided into six domains, namely, N-arm, P-domain, I-domain, A-domain, F-loop, and E-loop.

Article Snippet: The P22 phage (ATCC-97541-B1), which was purchased from the American Type Culture Collection (Manassas, VA, USA), was propagated on S. typhimurium for 4 h at 37 ◦C.

Techniques: Cryo-EM Sample Prep